Hi Janet,
I would like to thank you again for coming in to observe my students work on the crystallization lab/simulation I have learned over the summer at ASU. I have finally posted on my blog the entire activities that built up and relate to crystallization. I have spent a total of 150 hours with 120 hours in-class conducting the lessons and the labs and 30 hours of preparation to complete my commitment to the program.
Once again, thank you very much for the opportunity and I hope that I will have the chance to participate in similar programs in the future.
Sincerely,
Jannette O. Nuestro
Westwood High School
(480) 472-4616
Thursday, February 28, 2008
Tuesday, February 26, 2008
Protein Blogs Fall 2007
Fall Semester 2007 150 hours total (120 hours in-class and 30 hours of preparation for the lessons and labs)
3-Week Lesson on Crystallization of Protein (Based upon the Research on Photosynthesis/Crystallization of Protein with Dr. Petra Fromme)
Week 1 Protein in Relationship to Organic Compounds
9/17/07 Day 1 Notes on Organic Compounds
9/18/07 Day 2 Transparency on Life Molecules/Transparency on Classes of Macromolecules
9/19/07 Day 3 Powerpoint Lesson
9/20/07 Day 4 Powerpoint Lesson/Chapter Packet
9/21/07 Day 5 Lab Acitivity on Carbohydrates: Chemistry and Identification
=====================================================================================
Week 2 Lab Activities on Macromolecules
9/24/07 Day 1 Lab activity on Proteins
9/25/07 Day 2 Lab activity on Fat Molecules
9/26/07 Day 3 Organic Compound (Use of Models )
9/27/07 Day 4 Lab Activity Test for Proteins, Fat, Carbohydrates
9/28/07 Day 5 Chapter Assessment on Organic Compounds/Macromolecules
=====================================================================================
Week 3 Protein as an Important Organelle of the Cell/Plasma Membrane
10/03/07 Day 1 Transparency /Lecture on Plant and Animal Cells
10/04/07 Day 2 Plant and Animal Cell Identification of Parts and Function/Coloring Sheet
10/05/07 Day 3 Lab Activity on the Plasma Membrane and Membrane Proteins
12/13/07 Day 4 Crystallization Lab Part 1 (Lab Set-up and Formulate Hypothesis)
12/14/07 Day 5 Crystallization Lab Part 2 (Conclusion and Results)
I have applied the concept of Crystallization of protein I learned from Dr. Fromme’s lab to Organic compounds. Students were able to study in-depth the description, importance, structural , molecular formula and function of protein and other organic organic compounds in detail.
Day 1 Notes on Organic Compounds
This lesson was as an introduction to organic chemistry. Students were introduced to the properties of Carbon as an important element in organic compounds. They were able to gain background information on the different types of organic compounds: proteins, carbohydrates, fats and nucleic acids. Students worked quietly, have learned the procedure and know what was expected of them.
Day 2 Lecture/Transparency on Types of Macromolecules
This is a lecture type of activity where students were given the information on each macromolecule: proteins, lipids, carbohydrates, and nucleic acids. The lecture provided the description, function and examples for each macromolecules. Soon after the lecture the students gave the information back by answering questions orally in class and answering transparency questions on their own.
Day 3 and Day 4 Powerpoint Lessons
Students were given a powerpoint lesson on the different types of organic compounds. They were expected to fill in answers to the questions during the powerpoint lesson.
At the end of the 2-day lessons, the students were given a chapter packet which served as a study guide to reinforce what they have learned from the class.
Day 5 Lab Activity on Carbohydrates: Chemistry and Identification
Students learned how to write a molecular formula, structural formula for several carbohydrates. They have used the paper models to construct several types of carbohydrates. Students were prepared, given a background and procedure on how to work on their lab activity. They had a difficulty at the beginning but after following the procedures and using the paper models, they were able to work in pairs successfully. This lab activity came with a lab report question and answer type that they will need to complete while working with the lab.
Week 2
Day 1 Lab Acitivty on Proteins
Students worked on the proteins lab activity using paper models of protein, examine structural and molecular formulas of proteins, special arrangements, and combining amino acids to form proteins. Students were more comfortable with the use of paper models and were able to work independently.
Day 2 Lab Activity on Fats
Students were again given the paper models for fats, they studied the small molecules which join to make up fats, its chemical make-up and different types of fatty acids. This is the final activity using paper models for organic compounds. Students were asked to complete a table to compare and contrast the three macromolecules studied, carbohydrates, proteins and lipids.
Day 3 Organic Compounds (Use of Models)
Students were given a worksheet with tables, descriptions, and structural formulas to identify the organic compounds. They were also given the models to put together to form different organic compounds. My students really enjoyed this activity, had a clear understanding of the different elements that makes up each, and the similarities and differences in the structure, make-up and functions of each organic compound.
Day 4 Identifying Organic Compounds
Students have used the several indicators such as Biuret agent and Benedict’s solution to test for the presence of lipids, carbohydrates, and proteins in particular foods. Using simple chemical tests with substances called indicators, students will have to determine the presence of organic compounds. A color change of an indicator is usually a positive test for the presence of organic compound. Students were given their assigned groups and materials to work on. They completed a lab report and turned in at the end of the hour.
Day 5 Chapter Assessment
Students were assessed today after a two-week lesson of lecture and lab activities. Test questions were multiple choice questions to check up for student’s understanding of the unit.
Week 3
Day 1 transparency on Plant and Animal Cells
Students were given the transparency on plant and animal cells. They were asked to identify the different organelles within the eukaryotic cells. After identifying the different organelles, they were then asked to indicate the different functions of each organelle, compare and contrast between the two cells.
Day 2 Coloring Sheet on Plant and Animal Cells
This activity reinforced what my students have learned the previous day on eukaryotic cells. They were asked to identify using a coloring sheet the different organelles, provide its description and function of each.
Day 3 Lab Activity on Plasma Membrane and Membrane Proteins
Students worked on the structure of the plasma membrane, the membrane proteins embedded within, the function of the plasma membrane and the functions of the different types of protein. They were also asked to answer review questions based on the structure of the plasma membrane.
Day 4 Crystallization of Protein Part 1 (Lab Set-up and Formulate Hypothesis)
This is the first part of the lab where the students used the different problem solving techniques. The objective of this activity is for the students to create crystals that will simulate isolation of proteins which is a macromolecule from membrane of plants. Students were given the materials and before they started with their lab, they formulated their hypotheses on the growth of crystals based on the varying amounts of copper sulfate. After they have formulated their hypotheses, students then set-up the first part of their activity. They will need to let the crystals grow overnight and make observations the following day to check on the results of their lab preparation.
Day 5 Crystallization of Protein Part 2 (Observation and Conclusion)
Students made their observations on the growth of crystals and arrive at their conclusions. Their conclusions were based upon the growth of crystals and they have stated whether their hypotheses were correct or incorrect. Analysis questions relating to knowledge of macromolecules or organic compounds were also answered. My students were really excited about this activity that they even came in to check up on the growth of their crystals the same day they set-up their labs.
I would like to thank you again, Dr. Bond-Robinson for taking the time to come in to my classroom and observe how the students enjoy the lab on crystallization.
I have spent a total of 150 hours (120 hours of conducting the lessons/labs and another 30 hours for preparation).
3-Week Lesson on Crystallization of Protein (Based upon the Research on Photosynthesis/Crystallization of Protein with Dr. Petra Fromme)
Week 1 Protein in Relationship to Organic Compounds
9/17/07 Day 1 Notes on Organic Compounds
9/18/07 Day 2 Transparency on Life Molecules/Transparency on Classes of Macromolecules
9/19/07 Day 3 Powerpoint Lesson
9/20/07 Day 4 Powerpoint Lesson/Chapter Packet
9/21/07 Day 5 Lab Acitivity on Carbohydrates: Chemistry and Identification
=====================================================================================
Week 2 Lab Activities on Macromolecules
9/24/07 Day 1 Lab activity on Proteins
9/25/07 Day 2 Lab activity on Fat Molecules
9/26/07 Day 3 Organic Compound (Use of Models )
9/27/07 Day 4 Lab Activity Test for Proteins, Fat, Carbohydrates
9/28/07 Day 5 Chapter Assessment on Organic Compounds/Macromolecules
=====================================================================================
Week 3 Protein as an Important Organelle of the Cell/Plasma Membrane
10/03/07 Day 1 Transparency /Lecture on Plant and Animal Cells
10/04/07 Day 2 Plant and Animal Cell Identification of Parts and Function/Coloring Sheet
10/05/07 Day 3 Lab Activity on the Plasma Membrane and Membrane Proteins
12/13/07 Day 4 Crystallization Lab Part 1 (Lab Set-up and Formulate Hypothesis)
12/14/07 Day 5 Crystallization Lab Part 2 (Conclusion and Results)
I have applied the concept of Crystallization of protein I learned from Dr. Fromme’s lab to Organic compounds. Students were able to study in-depth the description, importance, structural , molecular formula and function of protein and other organic organic compounds in detail.
Day 1 Notes on Organic Compounds
This lesson was as an introduction to organic chemistry. Students were introduced to the properties of Carbon as an important element in organic compounds. They were able to gain background information on the different types of organic compounds: proteins, carbohydrates, fats and nucleic acids. Students worked quietly, have learned the procedure and know what was expected of them.
Day 2 Lecture/Transparency on Types of Macromolecules
This is a lecture type of activity where students were given the information on each macromolecule: proteins, lipids, carbohydrates, and nucleic acids. The lecture provided the description, function and examples for each macromolecules. Soon after the lecture the students gave the information back by answering questions orally in class and answering transparency questions on their own.
Day 3 and Day 4 Powerpoint Lessons
Students were given a powerpoint lesson on the different types of organic compounds. They were expected to fill in answers to the questions during the powerpoint lesson.
At the end of the 2-day lessons, the students were given a chapter packet which served as a study guide to reinforce what they have learned from the class.
Day 5 Lab Activity on Carbohydrates: Chemistry and Identification
Students learned how to write a molecular formula, structural formula for several carbohydrates. They have used the paper models to construct several types of carbohydrates. Students were prepared, given a background and procedure on how to work on their lab activity. They had a difficulty at the beginning but after following the procedures and using the paper models, they were able to work in pairs successfully. This lab activity came with a lab report question and answer type that they will need to complete while working with the lab.
Week 2
Day 1 Lab Acitivty on Proteins
Students worked on the proteins lab activity using paper models of protein, examine structural and molecular formulas of proteins, special arrangements, and combining amino acids to form proteins. Students were more comfortable with the use of paper models and were able to work independently.
Day 2 Lab Activity on Fats
Students were again given the paper models for fats, they studied the small molecules which join to make up fats, its chemical make-up and different types of fatty acids. This is the final activity using paper models for organic compounds. Students were asked to complete a table to compare and contrast the three macromolecules studied, carbohydrates, proteins and lipids.
Day 3 Organic Compounds (Use of Models)
Students were given a worksheet with tables, descriptions, and structural formulas to identify the organic compounds. They were also given the models to put together to form different organic compounds. My students really enjoyed this activity, had a clear understanding of the different elements that makes up each, and the similarities and differences in the structure, make-up and functions of each organic compound.
Day 4 Identifying Organic Compounds
Students have used the several indicators such as Biuret agent and Benedict’s solution to test for the presence of lipids, carbohydrates, and proteins in particular foods. Using simple chemical tests with substances called indicators, students will have to determine the presence of organic compounds. A color change of an indicator is usually a positive test for the presence of organic compound. Students were given their assigned groups and materials to work on. They completed a lab report and turned in at the end of the hour.
Day 5 Chapter Assessment
Students were assessed today after a two-week lesson of lecture and lab activities. Test questions were multiple choice questions to check up for student’s understanding of the unit.
Week 3
Day 1 transparency on Plant and Animal Cells
Students were given the transparency on plant and animal cells. They were asked to identify the different organelles within the eukaryotic cells. After identifying the different organelles, they were then asked to indicate the different functions of each organelle, compare and contrast between the two cells.
Day 2 Coloring Sheet on Plant and Animal Cells
This activity reinforced what my students have learned the previous day on eukaryotic cells. They were asked to identify using a coloring sheet the different organelles, provide its description and function of each.
Day 3 Lab Activity on Plasma Membrane and Membrane Proteins
Students worked on the structure of the plasma membrane, the membrane proteins embedded within, the function of the plasma membrane and the functions of the different types of protein. They were also asked to answer review questions based on the structure of the plasma membrane.
Day 4 Crystallization of Protein Part 1 (Lab Set-up and Formulate Hypothesis)
This is the first part of the lab where the students used the different problem solving techniques. The objective of this activity is for the students to create crystals that will simulate isolation of proteins which is a macromolecule from membrane of plants. Students were given the materials and before they started with their lab, they formulated their hypotheses on the growth of crystals based on the varying amounts of copper sulfate. After they have formulated their hypotheses, students then set-up the first part of their activity. They will need to let the crystals grow overnight and make observations the following day to check on the results of their lab preparation.
Day 5 Crystallization of Protein Part 2 (Observation and Conclusion)
Students made their observations on the growth of crystals and arrive at their conclusions. Their conclusions were based upon the growth of crystals and they have stated whether their hypotheses were correct or incorrect. Analysis questions relating to knowledge of macromolecules or organic compounds were also answered. My students were really excited about this activity that they even came in to check up on the growth of their crystals the same day they set-up their labs.
I would like to thank you again, Dr. Bond-Robinson for taking the time to come in to my classroom and observe how the students enjoy the lab on crystallization.
I have spent a total of 150 hours (120 hours of conducting the lessons/labs and another 30 hours for preparation).
Tuesday, July 17, 2007
July 16th blog
This is our last week in the research lab and Dr. Fromme wants us to try different lab activities that we can use in the classroom. She made several suggestions as to the type of materials that will be accessible for us and still be able to bring in the concept of crystallization in the classroom. Chemicals such as sucrose, sodium chloride, copper sulfate, and potassium chloride would be easy for us to find in our chemistry lab. We can use glass vials, we used wool, cotton, strand of hair, silicone and made several experimental set-ups. I am really excited about what we have tried because we can bring in knowledge from the research lab and connect it to the concept that we normally teach in the classroom such as photosynthesis.
July 13th blog
We spent most of our morning listening to a powerpoint prenetation of one of the grad students. We have seen three presentations the past 3 weeks and we can tell that this presnetation was so much better than the others. The student was better prepared, very confident about his data and very confident about his presentation. The rest of the time was spent with Petra helping us with our powerpoint presentation. She gave us a lot of suggestions on how to organize thoughts in our powerpoint. Monday, we will start with lab activities that we can use for our own classroom. We really have been fortunate to be assigned with Dr. Fromme's lab. She has been very helpful not only in the research lab but also would like for us to take activities learned from the lab to use in our classroom.
Thursday, July 12, 2007
July 12th blog
We looked at our PSI crystals by microdialysis tubing. These crystals are entirely different shape from all the other crystals we have looked at with different techniques. These crystals are longer and thinner and when magnified under the polarized dissecting scopes, they look like neon colored glow sticks - beautiful. We each tried to seed our crystals onto another microdialysis tubing by fishing for crystals. This was a very neat experience to try to transfer a dot-size crystals from one end to another tube :).
Wednesday, July 11, 2007
July 6th-11th blogs
July 9, 2007
We started today with a different crystallization technique of PS I called microdialysis tubing. Dr. Fromme mentioned that this type of technique is something that she shows her students the last due to the difficulty of the process. This involves the preparation of materials (microdialysis tube) and probably a more careful and steady hand specially when we get to trying to “fish” for crystals to be seeded. The next part of this technique will be continued on Thursday.
July 10th blog
We worked today with Dr. Wachter and her technician named Nam. Dr. Wachter gave a powerpoint presentation on PCR (polymerase chain reaction) and she gave us a little background on DNA. They are also involved in crystallization of protein called GFP (green fluorescent protein) from a jellyfish. After the powerpoint presentation, we started working on the process of PCR . We combine all the chemicals needed for PCR and centrifuge the PCR ingredients. We were given a chance to again use the tools that they use such as micropippetors , showed us how to use the PCR machine and how to prepare the agarose gell needed to show the movement of chemical (DNA). The lab we did today is a different experience which gave me a very neat experience and understanding of how DNA is used in PCR. This is an experience that I can take in the classroom as we are in the age of biotechnology.
July 11th blog
We started our day with placing the agarose gel in the electrophoresis chamber, transferred the PCR cocktail of chemicals we prepared yesterday and left it for an hour. Meanwhile, Dr. Wachter gave a powerpoint presentation on their goals and background on their research which is the GFP protein. It was good to be able to follow what was going on in the lab since I had a little bit of background on DNA J.
We started today with a different crystallization technique of PS I called microdialysis tubing. Dr. Fromme mentioned that this type of technique is something that she shows her students the last due to the difficulty of the process. This involves the preparation of materials (microdialysis tube) and probably a more careful and steady hand specially when we get to trying to “fish” for crystals to be seeded. The next part of this technique will be continued on Thursday.
July 10th blog
We worked today with Dr. Wachter and her technician named Nam. Dr. Wachter gave a powerpoint presentation on PCR (polymerase chain reaction) and she gave us a little background on DNA. They are also involved in crystallization of protein called GFP (green fluorescent protein) from a jellyfish. After the powerpoint presentation, we started working on the process of PCR . We combine all the chemicals needed for PCR and centrifuge the PCR ingredients. We were given a chance to again use the tools that they use such as micropippetors , showed us how to use the PCR machine and how to prepare the agarose gell needed to show the movement of chemical (DNA). The lab we did today is a different experience which gave me a very neat experience and understanding of how DNA is used in PCR. This is an experience that I can take in the classroom as we are in the age of biotechnology.
July 11th blog
We started our day with placing the agarose gel in the electrophoresis chamber, transferred the PCR cocktail of chemicals we prepared yesterday and left it for an hour. Meanwhile, Dr. Wachter gave a powerpoint presentation on their goals and background on their research which is the GFP protein. It was good to be able to follow what was going on in the lab since I had a little bit of background on DNA J.
July 6th blog
We spent our morning in three places, the X-ray lab, computer lab and conference room. We first looked at one of our lysozyme crystals setup wil liquid nitrogen ready for diffraction. The liquid nitrogen is necessary to prevent the protein from drying out. We then went to the computer lab to obtain data set of the diffraction pattern. Raimund Fromme then showed us how to obtain pretty ribbonlike form picture of the protein from the data set using a specific program. This part was really exciting because I have seen these protein pictures from Biology books but never really know how it was made. We then spent the second half of our morning listening to one of the grad student's presentation.
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